Dr.Aparachithudni on Instagram: "🧫 Gram Staining Process Used to differentiate bacteria into Gram-positive and Gram-negative based on cell wall structure. Steps: 1. Primary Stain – Crystal Violet → Stains all bacteria purple initially. 2. Mordant – Gram’s Iodine → Forms a crystal violet–iodine complex inside cells to strengthen the bond. 3. Decolorizer – Alcohol or Acetone → Gram-positive: Thick peptidoglycan retains the dye (stays purple) → Gram-negative: Thin peptidoglycan + outer membrane →