Nature

Development of a novel strategy to reduce diagnostic errors in real-time polymerase chain reaction using probe-based techniques

Real-time PCR assays are valuable tools for the rapid and accurate diagnosis of infectious diseases by identifying the nucleic acid sequences of pathogens. Here, we aimed to develop a recombinant ...
Nature

Real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of cassava brown streak viruses

Cassava brown streak disease (CBSD) caused by Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) is the most economically important viral disease of cassava. As cassava ...
News Medical

Diagnostics.ai launches fully-transparent machine learning platform for real-time PCR diagnostics

As the May 26th CE-IVDR compliance deadline comes into effect, Diagnostics.ai launches the industry's first fully-transparent machine learning platform for clinical real-time PCR diagnostics – ...

Ebola Outbreak: Why RT-PCR Is The Gold Standard For Testing Infections

According to WHO, it can be difficult to clinically distinguish Ebola disease from other infectious diseases such as malaria, typhoid fever, shigellosis, meningitis and other viral haemorrhagic fevers ...
The Scientist

How it Works: Real Time PCR

How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...